Journal: Alzheimer's Research & Therapy
Article Title: N-truncated Aβ 4–x peptides in sporadic Alzheimer’s disease cases and transgenic Alzheimer mouse models
doi: 10.1186/s13195-017-0309-z
Figure Lengend Snippet: Increased amyloid-β (Aβ) 4–x levels in homozygous 5XFAD mice a . Quantification of extracellular 029-2-positive plaque load in cortex, subiculum, and thalamus in heterozygous and homozygous 9-month-old 5XFAD mice. b Quantification of sodium dodecyl sulfate-soluble Aβ 4–40 levels in brains of heterozygous and homozygous 5-month-old 5XFAD mice revealed a significant gene dose-dependent increase. *** p < 0.001; * p < 0.05. ELISA Enzyme-linked immunosorbent assay, FAD Familial Alzheimer’s disease
Article Snippet: Subsequently, 50 μl of trimethylbenzidine ELISA peroxidase substrate (Interchim, Montluçon, France) was added and incubated for 1–5 minutes at RT in the dark.
Techniques: Enzyme-linked Immunosorbent Assay